Publications by Ehsan Razmara Monash Uni

venn graph

19.05.2022

library(ggplot2) library(ggvenn) ## Loading required package: dplyr ## ## Attaching package: 'dplyr' ## The following objects are masked from 'package:stats': ## ## filter, lag ## The following objects are masked from 'package:base': ## ## intersect, setdiff, setequal, union ## Loading required package: grid library(ggVennDiagram) gene...

22 sym R (840 sym/13 pcs) 4 img

radar_plot

19.05.2022

scores <- data.frame( row.names = c("Naive CD4 T", "CD14+ Mono" , "Memory CD4 T"), LDHB = c(7.9, 3.9, 9.4), S100A9 = c(10, 20, 0), IL32 = c(3.7, 11.5, 2.5), CD79A = c(8.7, 20, 4), CCL5 = c(7.9, 7.2, 12.4), MS4A7 = c(6.4, 10.5, 6.5), GZMB = c(2.4, 0.2, 9.8), FCER1A = c(0, 0, 20), PF4 =...

19 sym R (2352 sym/11 pcs) 3 img

raceid

19.05.2022

library(RaceID) library(Seurat) ## Attaching SeuratObject library(dplyr) ## ## Attaching package: 'dplyr' ## The following objects are masked from 'package:stats': ## ## filter, lag ## The following objects are masked from 'package:base': ## ## intersect, setdiff, setequal, union library(ggplot2) pbmc <- readRDS("/mnt/nectar_volume/hom...

2688 sym R (49104 sym/55 pcs) 26 img

volcano_seurat

17.05.2022

library(Seurat) ## Attaching SeuratObject library(SeuratWrappers) library(ggplot2) library(dplyr) ## ## Attaching package: 'dplyr' ## The following objects are masked from 'package:stats': ## ## filter, lag ## The following objects are masked from 'package:base': ## ## intersect, setdiff, setequal, union pbmc_tutorial_final <- readRDS(...

120 sym R (5260 sym/14 pcs) 1 img

heatmap enhancement

17.05.2022

library(RColorBrewer) library(Seurat) ## Attaching SeuratObject library(dplyr) ## ## Attaching package: 'dplyr' ## The following objects are masked from 'package:stats': ## ## filter, lag ## The following objects are masked from 'package:base': ## ## intersect, setdiff, setequal, union library(RColorBrewer) library(pheatmap) library(Co...

42 sym R (6435 sym/33 pcs) 4 img

cell cycle regression

06.05.2022

As the "cc.genes$s.genes/g2m.genes have been written for Human, we are supposed to change the organism here, i.e., we need to align all genes in mice. To this end, we should use the following functions. library(biomaRt) library(Seurat) ## Attaching SeuratObject library(org.Hs.eg.db) ## Loading required package: AnnotationDbi ## Loading required p...

637 sym R (23125 sym/58 pcs) 6 img

Liger

05.05.2022

library(rliger) ## Loading required package: cowplot ## Loading required package: Matrix ## Loading required package: patchwork ## ## Attaching package: 'patchwork' ## The following object is masked from 'package:cowplot': ## ## align_plots library(Seurat) ## Attaching SeuratObject library(SeuratData) ## ── Installed datasets ───�...

152 sym R (14545 sym/51 pcs) 1 img

Seurat obj batch effect elimination

04.05.2022

library(Seurat) ## Attaching SeuratObject library(SeuratData) ## ── Installed datasets ───────────────────────────────────── SeuratData v0.2.2 ── ## ✓ ifnb 3.1.0 ✓ pbmcsca 3.0.0 ## ✓ panc8 3.0.2 ## ───────────...

292 sym R (4244 sym/31 pcs) 1 img

harmony batch effect elimination

05.05.2022

Herein, we will utilise the Signac R package’s Harmony batch effect correction method (Korsunsky et al. 2019). The Harmony algorithm is accessible on GitHub, and Signac provides integration tutorials. library(dplyr) ## ## Attaching package: 'dplyr' ## The following objects are masked from 'package:stats': ## ## filter, lag ## The followi...

886 sym R (5671 sym/66 pcs) 2 img

seurat stacked vlnplot

10.05.2022

library(Seurat) ## Attaching SeuratObject library(SeuratWrappers) library(ggplot2) library(dplyr) ## ## Attaching package: 'dplyr' ## The following objects are masked from 'package:stats': ## ## filter, lag ## The following objects are masked from 'package:base': ## ## intersect, setdiff, setequal, union pbmc_tutorial_final <- readRDS(...

120 sym R (5260 sym/14 pcs) 1 img